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BIBTEX RIS

The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] Assay Is a Fast and Reliable Method for Colorimetric Determination of Fungal Cell Densities

1999; American Society for Microbiology; Volume: 65; Issue: 8 Linguagem: Inglês

10.1128/aem.65.8.3727-3729.1999

ISSN

1098-5336

Autores

Florian M. Freimoser, Claude A. Jakob, Markus Aebi, Urs Tuor,

Tópico(s)

Analytical Chemistry and Chromatography

Resumo

ABSTRACT The entomopathogenic fungus Neozygites parvispora (Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies in a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for the cultivation of this fungus, we were looking for a rapid and quantitative method to estimate the number of living cells in small volumes of liquid culture. A colorimetric method for the determination of cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] proved to be more accurate and timesaving than conventional hemocytometer counting.

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