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Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

2010; Wiley; Volume: 60; Issue: 2 Linguagem: Inglês

10.1111/j.1365-3059.2010.02367.x

1365-3059

Sahar Eid, Dayle E. Saar, K. L. Druffel, H. R. Pappu,

Plant Parasitism and Resistance

Selected wild Dahlia species in their natural habitats from west‐central Mexico were tested for the presence of three caulimoviruses known to be associated with cultivated dahlia ( Dahlia variabilis ), viz. Dahlia mosaic virus (DMV), DMV‐D10 and Dahlia common mosaic virus. Virus species‐specific primers and PCR were used followed by cloning and sequencing of the amplicons. Results showed that the wild dahlia species in their natural habitat contained DMV‐D10, which is an endogenous plant pararetrovirus. Viral sequences were found in 91% of the samples ( n = 56) representing four different wild species. The gene coding for the movement protein of DMV‐D10 from Dahlia coccinea and all other species was cloned and sequenced. Sequence comparisons showed divergence of this gene when compared to that of DMV‐D10 from cultivated dahlias. The discovery of plant pararetroviruses in wild dahlia species in their natural habitats suggests a possible emergence, co‐existence and co‐evolution of pararetroviruses and their host plants.