Evaluation of antifungal herbs on filamentous fungi using a rapid screening technique (657.7)
2014; Wiley; Volume: 28; Issue: S1 Linguagem: Inglês
10.1096/fasebj.28.1_supplement.657.7
ISSN1530-6860
AutoresBrian Phung, Aaron Ahn, Kim HyeSoo, Edwin S. Bennett, Erica A. Fradinger, Priscilla Bell,
Tópico(s)Plant Pathogens and Fungal Diseases
ResumoPhytochemicals have been studied as antifungal agents due to the growing resistance of fungi to current medications. The demand of these reagents has increased due to the rising in number of immune‐deficient patients worldwide. Current methods for screening antifungal herbs involve the lengthy CLSI method (M38‐A2). Here we used a novel rapid screening technique to evaluate the inhibition of filamentous fungal growth by Bayberry Root, Elecampane, Goldenrod, and Wood Betony. Filamentous fungi were grown on potato dextrose agar plates and ground in Delbecco Medium with Fetal Bovine Serum. The slurry was filtered using a #1 Whatman filter and used for testing with the various herb preparations. Herbs were extracted with methanol, filtered, rotoevaporated, and then diluted in DMSO. Initial transmittance at 625 nm was taken after preparation and subsequent readings were taken hourly after overnight incubation at 37 ° C. Bayberry Root, Elecampane, Goldenrod, and Wood Betony yielded inhibition percentages of 32.1 ± 3.7, 47.1 ± 7.9, 34.5 ± 15.8, and 10.0 ± 4.7 respectively, at 50% growth of the control fungal preparation. These data demonstrate that our herb preparations show significant inhibition of fungal growth and this study will be expanded to include related filamentous fungi, as well as, additional herbs.
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