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Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions.

1994; Springer Nature; Volume: 13; Issue: 24 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06960.x

1460-2075

Gary J. Sharples, Sau N. Chan, Akeel A. Mahdi, Matthew C. Whitby, Robert G. Lloyd,

DNA and Nucleic Acid Chemistry

Research Article15 December 1994free access Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. G.J. Sharples G.J. Sharples Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author S.N. Chan S.N. Chan Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author A.A. Mahdi A.A. Mahdi Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author M.C. Whitby M.C. Whitby Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author R.G. Lloyd R.G. Lloyd Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author G.J. Sharples G.J. Sharples Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author S.N. Chan S.N. Chan Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author A.A. Mahdi A.A. Mahdi Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author M.C. Whitby M.C. Whitby Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author R.G. Lloyd R.G. Lloyd Department of Genetics, University of Nottingham, Queens Medical Centre, UK. Search for more papers by this author Author Information G.J. Sharples1, S.N. Chan1, A.A. Mahdi1, M.C. Whitby1 and R.G. Lloyd1 1Department of Genetics, University of Nottingham, Queens Medical Centre, UK. The EMBO Journal (1994)13:6133-6142https://doi.org/10.1002/j.1460-2075.1994.tb06960.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The formation and subsequent resolution of Holliday junctions are critical stages in recombination. We describe a new Escherichia coli endonuclease that resolves Holliday intermediates by junction cleavage. The 14 kDa Rus protein binds DNA containing a synthetic four-way junction (X-DNA) and introduces symmetrical cuts in two strands to give nicked duplex products. Rus also processes Holliday intermediates made by RecA into products that are characteristic of junction resolution. The cleavage activity on X-DNA is remarkably similar to that of RuvC. Both proteins preferentially cut the same two strands at the same location. Increased expression of Rus suppresses the DNA repair and recombination defects of ruvA, ruvB and ruvC mutants. We conclude that all ruv strains are defective in junction cleavage, and discuss pathways for Holliday junction resolution by RuvAB, RuvC, RecG and Rus. Previous ArticleNext Article Volume 13Issue 241 December 1994In this issue RelatedDetailsLoading ...