Assessment of cross-taxa utility of coconut microsatellite markers.
2008; Indian Academy of Horticulture Sciences; Volume: 65; Issue: 3 Linguagem: Inglês
ISSN
0972-8538
AutoresN. Anitha, K. Lakshmi Jayaraj, E. P. A. Kumar, Jiji George, M. K. Rajesh,
Tópico(s)Cassava research and cyanide
ResumoMicro-satellite or simple sequence repeats (SSRs) are abundant across eukaryotic genomes and show high levels of polymorphism. Micro-satellite DNA often has flanking regions that are highly conserved in related species and this renders the primer pairs designed in one species useful for the amplification of the same DNA region in related genomes, minimizing laborious cloning and screening steps. The present investigation explored the transferability of coconut SSRs to other palms, viz., oil palm, arecanut, palmyrah and date palm. The annealing temperature of 86 coconut-specific SSR primers was standardized using gradient PCR. Of the 86 primers, 55 primers gave clear bands of expected size range (100 to 300 bp) and these were tested for their cross-taxa amplification. The percentage of cross-amplification of coconut SSR loci were 36.36% in oil palm, 29.09% in arecanut, 18.18% in palmyrah and 12.70% in date palm. The results suggest usefulness of coconut SSRs for phylogenetic and comparative genomic studies in other palms.
Referência(s)