Produção Nacional
Revisado por pares
Analysis of a Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) for yellow fever diagnostic
2015; Elsevier BV; Volume: 226; Linguagem: Inglês
10.1016/j.jviromet.2015.10.003
ISSN1879-0984
AutoresMárcio Roberto Teixeira Nunes, João Lídio da Silva Gonçalves Vianez Júnior, Keley N.B. Nunes, Sandro Patroca da Silva, Clayton Pereira Silva de Lima, Hilda Guzmán, Livia Carício Martins, Valéria Lima Carvalho, Robert B. Tesh, Pedro Fernando da Costa Vasconcelos,
Tópico(s)Viral Infections and Vectors
ResumoYellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi), as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.
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