Portal de Periódicos da CAPES

Splicing of cauliflower mosaic virus 35S RNA is essential for viral infectivity.

1995; Springer Nature; Volume: 14; Issue: 14 Linguagem: Inglês

10.1002/j.1460-2075.1995.tb07361.x

1460-2075

Z. Kiss-László, Stéphane Blanc, T. Höhn,

Plant-Microbe Interactions and Immunity

Research Article17 July 1995free access Splicing of cauliflower mosaic virus 35S RNA is essential for viral infectivity. Z. Kiss-László Z. Kiss-László Friedrich Miescher Institute, Basel, Switzerland. Search for more papers by this author S. Blanc S. Blanc Friedrich Miescher Institute, Basel, Switzerland. Search for more papers by this author T. Hohn T. Hohn Friedrich Miescher Institute, Basel, Switzerland. Search for more papers by this author Z. Kiss-László Z. Kiss-László Friedrich Miescher Institute, Basel, Switzerland. Search for more papers by this author S. Blanc S. Blanc Friedrich Miescher Institute, Basel, Switzerland. Search for more papers by this author T. Hohn T. Hohn Friedrich Miescher Institute, Basel, Switzerland. Search for more papers by this author Author Information Z. Kiss-László1, S. Blanc1 and T. Hohn1 1Friedrich Miescher Institute, Basel, Switzerland. The EMBO Journal (1995)14:3552-3562https://doi.org/10.1002/j.1460-2075.1995.tb07361.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A splicing event essential for the infectivity of a plant pararetrovirus has been characterized. Transient expression experiments using reporter constructs revealed a splice donor site in the leader sequence of the cauliflower mosaic virus (CaMV) 35S RNA and three additional splice donor sites within open reading frame (ORF) I. All four donors use the same splice acceptor within ORF II. Splicing between the leader and ORF II produces an mRNA from which ORF III and, in the presence of the CaMV translational transactivator, ORF IV can be translated efficiently. The other three splicing events produce RNAs encoding ORF I-II in-frame fusions. All four spliced CaMV RNAs were detected in CaMV-infected plants. Virus mutants in which the splice acceptor site in ORF II is inactivated are not infectious, indicating that splicing plays an essential role in the CaMV life cycle. The results presented here suggest a model for viral gene expression in which RNA splicing is required to provide appropriate substrate mRNAs for the specialized translation mechanisms of CaMV. Previous ArticleNext Article Volume 14Issue 141 July 1995In this issue RelatedDetailsLoading ...