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First Report of Anthracnose on Chayote Fruits ( Sechium edule ) Caused by Colletotrichum brevisporum
2015; American Phytopathological Society; Volume: 100; Issue: 1 Linguagem: Inglês
10.1094/pdis-07-15-0793-pdn
ISSN1943-7692
AutoresJuliane Bezerra, Paulo Vanderlei Ferreira, Leonardo da Fonseca Barbosa, Roberto Ramos‐Sobrinho, Danilo Batista Pinho, Ailton Reis, Iraíldes Pereira Assunção, Gaus Silvestre de Andrade Lima,
Tópico(s)Plant Pathogens and Resistance
ResumoHomePlant DiseaseVol. 100, No. 1First Report of Anthracnose on Chayote Fruits (Sechium edule) Caused by Colletotrichum brevisporum PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Anthracnose on Chayote Fruits (Sechium edule) Caused by Colletotrichum brevisporumJ. P. Bezerra, P. V. Ferreira, L. da F. Barbosa, R. Ramos-Sobrinho, D. B. Pinho, A. Reis, I. P. Assunção, and G. S. A. LimaJ. P. BezerraSearch for more papers by this author, P. V. FerreiraSearch for more papers by this author, L. da F. BarbosaSearch for more papers by this author, R. Ramos-SobrinhoSearch for more papers by this author, D. B. PinhoSearch for more papers by this author, A. ReisSearch for more papers by this author, I. P. AssunçãoSearch for more papers by this author, and G. S. A. LimaSearch for more papers by this authorAffiliationsAuthors and Affiliations J. P. Bezerra , Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, AL, Brazil, 57072-970, and Setor de Fitossanidade/Centro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL, Brazil, 57100-000 P. V. Ferreira , Setor de Melhoramento Genético de Plantas/Centro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL, Brazil, 57100-000 L. da F. Barbosa R. Ramos-Sobrinho , Setor de Fitossanidade/Centro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL, Brazil, 57100-000 D. B. Pinho , Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, MG, Brazil, 36570-900 A. Reis , Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Hortaliças, Brasília, DF, Brazil, 70351-970 I. P. Assunção G. S. A. Lima , Setor de Fitossanidade/Centro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL, Brazil, 57100-000. Published Online:25 Nov 2015https://doi.org/10.1094/PDIS-07-15-0793-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Colletotrichum orbiculare is traditionally considered the etiological agent of anthracnose on such Cucurbitaceae as chayote, cucumber, melons, pumpkin, squash, and watermelon (Tocchetto 1946; Damm et al. 2013). Recently, an isolate of Colletotrichum with morphological characteristics distinct from C. orbiculare was collected from chayote fruits in Antonio Carlos, Santa Catarina State, Brazil (27°30′43″ S; 48°46′18″ W). Therefore, this study aimed to verify the etiology of anthracnose on chayote (Sechium edule) based on morphological and molecular data. The symptoms initially appear as soaked and cylindrical lesions on the surface of the fruit, which gradually enlarges in size, becomes elliptical, sunken and pale brown to black. A monosporic isolate was obtained on potato dextrose agar (PDA), and derived culture was deposited in the Coleção de Culturas de Fungos Fitopatogênicos da Universidade Federal de Alagoas (code COUFAL7300). Colonies on PDA were white to off-white, cottony to felted; and in reverse uniformly white to pale becoming orange toward the center with a growth rate of 6.61 mm/day and sparse sporulation. The hyaline, aseptate, cylindrical with round ends, smooth-walled, guttulate conidia were 7.5 to 10.5 µm long and 2 to 3 µm wide, differing from the morphological characteristics of C. orbiculare (10.5 to 12.5 µm long and 4 to 4.5 µm wide). For precise identification, partial sequences of the actin (ACT), calmodulin (CAL), chitin synthase (CHS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), internal transcribed spacer (ITS), and β-tubulin 2 (β-TUB) genomic regions were obtained and deposited in GenBank (Accession Nos. KT285378 to KT285383). The sequence of the ACT and other regions (CAL, CHS, GAPDH, ITS, and β-TUB) exhibited 100% and 99% of identity, respectively, with sequences from C. brevisporum strain GZAAS5.09545. Additionally, Bayesian inference analysis using concatenate sequences (ACT, CAL, CHS, GAPDH, ITS, and β-TUB) placed our isolate in a same clade with others C. brevisporum (study S17963 deposited in TreeBASE). To confirm pathogenicity, mycelial PDA discs of 5 mm were taken from a 7-day-old culture and deposited on five mature chayote fruits superficially wounded with a sterilized needle. In control fruits, only PDA discs without fungal growth were used. Fruits were kept for two days in a humid chamber at 25°C. This pathogenicity test was additionally carried out using commercial (Acclaim, Syngenta) and noncommercial fruits of Cucumis melo from the Melon Breeding Program of UFAL. After six days, soaked and pale-to-black, sunken lesions were observed on 100% of the inoculated fruits. No disease symptom was observed on the control fruits. To fulfill Koch’s postulates, the fungus C. brevisporum was successfully reisolated from inoculated chayote and melon fruits. This pathogen has been previously reported in Thailand and Korea in association with Neoregalia spp., Pandanus pygmaeus, and Lycium chinense (Noireung et al. 2012; Paul et al. 2014), and recently reported on papaya in Brazil (Vieira et al. 2013). To our knowledge, this is the first report of C. brevisporum causing anthracnose on chayote fruits worldwide.References:Damm, U. 2013. Fungal Divers. 61:29. https://doi.org/10.1007/s13225-013-0255-4 Crossref, ISI, Google ScholarNoireung, P. 2012. Cryptogam., Mycol. 33:347. https://doi.org/10.7872/crym.v33.iss3.2012.347 Crossref, ISI, Google ScholarPaul, N. C. 2014. Int. J. Mol. Sci. 15:15272. https://doi.org/10.3390/ijms150915272 Crossref, ISI, Google ScholarTocchetto, A. Chayote anthracnose. Bol. agron., 1946. Google ScholarVieira, W. A. S. 2013. Plant Dis. 97:1659. https://doi.org/10.1094/PDIS-05-13-0520-PDN Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 1 January 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 15 Jan 2016Published: 25 Nov 2015First Look: 10 Sep 2015Accepted: 1 Sep 2015 Pages: 217-217 Information© 2016 The American Phytopathological SocietyCited byColletotrichum brevisporumCABI Compendium, Vol. CABI CompendiumOrigin, Evolution, Breeding, and Omics of Chayote, an Important Cucurbitaceae Vegetable Crop24 September 2021 | Frontiers in Plant Science, Vol. 12Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1Brevianthrones, bianthrones from a Chinese isolate of the endophytic fungus Colletotrichum brevisporumPhytochemistry, Vol. 188Identification of Colletotrichum brevisporum causing fruit rot in yellow passion fruit (Passiflora edulis f. flavicarpa) in China15 January 2021 | Australasian Plant Pathology, Vol. 50, No. 2Analysis of genetic diversity of Colletotrichum population causing anthracnose in fruit crops using ISSR markers27 November 2020 | Indian Phytopathology, Vol. 74, No. 1Diversity of endophytic fungi associated with Carapichea ipecacuanha from a native fragment of the Atlantic Rain ForestSouth African Journal of Botany, Vol. 134Characterization, Phylogenetic Analyses, and Pathogenicity of Colletotrichum Species on Morus alba in Sichuan Province, ChinaLonghai Xue, Lei Zhang, Xiao Xiang Yang, Xiaoqin Huang, Wenxian Wu, Xiquan Zhou, James F. 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