Portal de Periódicos da CAPES

Regulation of expression of the lung amiloride-sensitive Na+ channel by steroid hormones.

1994; Springer Nature; Volume: 13; Issue: 9 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06494.x

1460-2075

Guy Champigny, Nicolas Voilley, Éric Lingueglia, Valérie Friend, Pascal Barbry, Michel Lazdunski,

Neonatal Respiratory Health Research

Research Article1 May 1994free access Regulation of expression of the lung amiloride-sensitive Na+ channel by steroid hormones. G. Champigny G. Champigny Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author N. Voilley N. Voilley Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author E. Lingueglia E. Lingueglia Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author V. Friend V. Friend Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author P. Barbry P. Barbry Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author M. Lazdunski M. Lazdunski Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author G. Champigny G. Champigny Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author N. Voilley N. Voilley Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author E. Lingueglia E. Lingueglia Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author V. Friend V. Friend Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author P. Barbry P. Barbry Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author M. Lazdunski M. Lazdunski Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. Search for more papers by this author Author Information G. Champigny1, N. Voilley1, E. Lingueglia1, V. Friend1, P. Barbry1 and M. Lazdunski1 1Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. The EMBO Journal (1994)13:2177-2181https://doi.org/10.1002/j.1460-2075.1994.tb06494.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Molecular cloning of the amiloride-sensitive Na+ channel has permitted analysis of the mechanisms of its stimulation by steroids. In rat lung cells in primary culture, where its mRNA has been detected, the activity of an amiloride-sensitive channel, highly selective for Na+, is controlled by corticosteroids. Dexamethasone (0.1 microM) or aldosterone (1 microM) induced, after a minimum 10 h treatment, a large increase of the amiloride-induced hyperpolarization and of the amiloride-sensitive current. A parallel increase in the amount of the mRNA was observed. The corresponding gene is thus a target for steroid action. Using synthetic specific agonists and antagonists for mineralo- and glucocorticoid receptors, it has been shown that the steroid action on Na+ channel expression is mediated via glucocorticoid receptors. Triiodothyronine, known to modulate steroid action in several tissues, had no effect on both the amiloride-sensitive Na+ current and the level of the mRNA for the Na+ channel protein, but potentiates the stimulatory effect of dexamethasone. The increase in Na+ channel activity observed in the lung around birth can thus be explained by a direct increase in transcription of the Na+ channel gene. Previous ArticleNext Article Volume 13Issue 91 May 1994In this issue RelatedDetailsLoading ...