Molecular basis of two subfamilies of immunoglobulin-like chaperones.
1996; Springer Nature; Volume: 15; Issue: 15 Linguagem: Inglês
10.1002/j.1460-2075.1996.tb00753.x
ISSN1460-2075
AutoresDanielle L. Hung, Stefan D. Knight, Roy Woods, Jerome S. Pinkner, Scott J. Hultgren,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoResearch Article1 August 1996free access Molecular basis of two subfamilies of immunoglobulin-like chaperones. D. L. Hung D. L. Hung Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author S. D. Knight S. D. Knight Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author R. M. Woods R. M. Woods Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author J. S. Pinkner J. S. Pinkner Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author S. J. Hultgren S. J. Hultgren Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author D. L. Hung D. L. Hung Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author S. D. Knight S. D. Knight Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author R. M. Woods R. M. Woods Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author J. S. Pinkner J. S. Pinkner Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author S. J. Hultgren S. J. Hultgren Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. Search for more papers by this author Author Information D. L. Hung1, S. D. Knight1, R. M. Woods1, J. S. Pinkner1 and S. J. Hultgren1 1Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA. The EMBO Journal (1996)15:3792-3805https://doi.org/10.1002/j.1460-2075.1996.tb00753.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The initial encounter of a microbial pathogen with the host often involves the recognition of host receptors by different kinds of bacterial adhesive organelles called pili, fimbriae, fibrillae or afimbrial adhesins. The development of over 26 of these architecturally diverse adhesive organelles in various Gram-negative pathogens depends on periplasmic chaperones that are comprised of two immunoglobulin-like domains. All of the chaperones possess a highly conserved sheet in domain 1 and a conserved interdomain hydrogen-bonding network. Chaperone-subunit complex formation depends on the anchoring of the carboxylate group of the subunit into the conserved crevice of the chaperone cleft and the subsequent positioning of the COOH terminus of subunits along the exposed edge of the conserved sheet of the chaperone. We discovered that the chaperones can be divided into two distinct subfamilies based upon conserved structural differences that occur in the conserved sheet. Interestingly, a subdivision of the chaperones based upon whether they assemble rod-like pili or non-pilus organelles that have an atypical morphology defines the same two subgroups. The molecular dissection of the two chaperone subfamilies and the adhesive fibers that they assemble has advanced our understanding of the development of virulence-associated organelles in pathogenic bacteria. Previous ArticleNext Article Volume 15Issue 151 August 1996In this issue RelatedDetailsLoading ...
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