Subtyping Analysis of Fanconi Anemia by Immunoblotting and Retroviral Gene Transfer
1998; BioMed Central; Volume: 4; Issue: 7 Linguagem: Inglês
10.1007/bf03401752
ISSN1528-3658
AutoresMichael A. Pulsipher, Gary M. Kupfer, Dieter Näf, Ahmed Suliman, Jeng-Shin Lee, Petra Jakobs, Markus Grompe, Hans Joenje, Colin A. Sieff, Eva C. Guinan, Richard C. Mulligan, Alan D. D’Andrea,
Tópico(s)Chromosomal and Genetic Variations
ResumoFanconi anemia (FA) is an autosomal recessive cancer susceptibility syndrome with at least eight complementation groups (A–H). Two of the FA genes (FAA and FAC) have been cloned, and mutations in these genes account for approximately 80% of FA patients. Subtyping of FA patients is an important first step toward identifying candidates for FA gene therapy. In the current study, we analyzed a reference group of 26 FA patients of known subtype. Most of the patients (18/26) were confirmed as either type A or type C by immunoblot analysis with anti-FAA and anti-FAC antisera. In order to resolve the subtype of the remaining patients, we generated retroviral constructs expressing FAA and FAC for transduction of FA cell lines (pMMP-FAA and pMMP-FAC). The pMMP-FAA construct specifically complemented the abnormal phenotype of cell lines from FA-A patients, while pMMP-FAC complemented FA-C cells. In summary, the combination of immunoblot analysis and retroviral-mediated phenotypic correction of FA cells allows a rapid method of FA subtyping.
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