In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteins The GenBank accession number for EcOYW1 is AB010426.
2001; Microbiology Society; Volume: 147; Issue: 2 Linguagem: Inglês
10.1099/00221287-147-2-507
ISSN1465-2080
AutoresHisashi Nishigawa, Shin-ichi Miyata, Kenro Oshima, Toshimi Sawayanagi, Akihiro Komoto, Tsutomu Kuboyama, Izumi Matsuda, Tsuneo Tsuchizaki, S. Namba,
Tópico(s)Cocoa and Sweet Potato Agronomy
ResumoA new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M, a mild symptom line derived from OY-W. We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 7·0 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes.
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