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Human Arylhydrocarbon Receptor: Functional Expression and Chromosomal Assignment to 7p211

1994; Oxford University Press; Volume: 116; Issue: 4 Linguagem: Inglês

10.1093/oxfordjournals.jbchem.a124605

1756-2651

Masatsugu Ema, Natsuki Matsushita, Kazuhiro Sogawa, Takeshi Ariyama, Johji Inazawa, Takayuki Nemoto, Minoru Ota, Mitsuo Oshimura, Yoshiaki Fujii-Kuriyama,

Drug Transport and Resistance Mechanisms

We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. The overall amino acid identity with mouse AhR from cell line Hepa-1 is 72.5%. The human AhR expressed either in COS-7 cells or in a reticulocyte lysate in vitro translation system showed specific dioxin-binding activity and Arnt-dependent DNA-binding activity. Chromosomal localization of the AhR gene was determined to be chromosome 7p21 by fluorescent in situ hybridization and DNA blot hybridization using 23 human x mouse or Chinese hamster hybrid cell DNAs.