Portal de Periódicos da CAPES

Monoclonal Antibodies Specific for Non-Derivatized Gibberellins I. Preparation of Monoclonal Antibodies against GA₄ and Their Use in Immunoaffinity Column Chromatography

1991; Oxford University Press; Linguagem: Inglês

10.1093/oxfordjournals.pcp.a078110

1471-9053

Masatoshi Nakajima, Isomaro Yamaguchi, Akira Nagatani, Satoru Kizawa, Noboru Murofushi, Masaki Furuya, Nobutaka Takahashi,

Mycotoxins in Agriculture and Food

Cell lines producing monoclonal antibodies specific for non-derivatized gibberellins (GAs) were prepared from spleen cells of a mouse immunized with an immunogen that carried 16-substituted GA4 as a hapten. The conditions for association and dissociation of antibody-hapten complexes were examined to determine the most effective procedure for immunoaffinity chromatography. The final procedure adopted was proven to be very effective by its application to the analysis of GAs in the anthers of rice and immature seeds of Phaseolus vulgaris