[116] Neuraminidase (sialidase) from influenza virus
1966; Academic Press; Linguagem: Inglês
10.1016/0076-6879(66)08122-9
ISSN1557-7988
AutoresMax E. Rafelson, Sondra Gold, Inna Priede,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoThis chapter discusses the synthesis of neuraminidase from influenza virus. N-Acetylneuraminic acid (NANA) liberated by enzymatic action from a susceptible glycoside of NANA is determined by the thiobarbituric acid colorimetric procedure. The preferred substrate is N-acetyl neuraminyllactose (NAN-LAC), which is better characterized and more easily obtained than glycoprotein substrates. The reagents used, preparation procedure followed, and the steps involved in the purification are also described in the chapter. The method described in the chapter is applicable to all strains of influenza virus studied; the Jap 305 strain of influenza virus is one of the choices because of its high content of neuraminidase. The Jap 305 strain of influenza virus is propagated in 10-day-old embryonated eggs by the inoculation of 0.1 ml of a 1:1000 dilution of infective chorioallantoic fluid per egg. The enzyme preparation is homogeneous in the ultracentrifuge. The enzyme solution may be stored indefinitely at 4° or lyophilized after dialysis against distilled water without appreciable loss of activity.
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