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BIBTEX RIS

Transformation of Actinomyces spp. by a gram-negative broad-host-range plasmid

1994; American Society for Microbiology; Volume: 176; Issue: 13 Linguagem: Inglês

10.1128/jb.176.13.4173-4176.1994

ISSN

1098-5530

Autores

M K Yeung, Cheri S. Kozelsky,

Tópico(s)

Genomics and Phylogenetic Studies

Resumo

The gram-negative broad-host-range vector pJRD215 was transferred by electroporation into strains of Actinomyces viscosus or Actinomyces naeslundii at efficiencies which ranged from 10(2) to 10(7) transformants per microgram of plasmid DNA. The Actinomyces transformants expressed pJRD215-encoded resistance to kanamycin and streptomycin. Moreover, the transforming plasmid DNA had not undergone any deletions or rearrangements, nor had it integrated into the genomes of these strains.

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