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Morphological Description of Transgenic Soybean Chimeras Created by the Delivery, Integration and Expression of Foreign DNA Using Electric Discharge Particle Acceleration

1990; Oxford University Press; Volume: 66; Issue: 4 Linguagem: Inglês

10.1093/oxfordjournals.aob.a088039

1095-8290

Paul Christou,

Plant Genetic and Mutation Studies

Transgenic soybean (Glycine max L.) plants derived from electric discharge particle acceleration experiments exhibited varying degrees of chimerism which was followed by the expression of the introduced β-glucuronidase (gus) gene. Degrees of chimerism in transgenic plants were established by determining expression of the gus gene observed as blue spots, streaks or sectors in stem and leaf tissues in in vitro grown plantlets and greenhouse plants. Clonal plants were also obtained. Presence of the gene was confirmed by Southern blot analysis. These studies permitted the reconstruction of a partial picture for the development of the soybean plant.