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BIBTEX RIS

Detection of dengue virus type 2 in Aedes albopictus by nucleic acid hybridization with strand-specific RNA probes

1988; American Society for Microbiology; Volume: 26; Issue: 3 Linguagem: Inglês

10.1128/jcm.26.3.579-581.1988

ISSN

1098-660X

Autores

Kenneth E. Olson, Carol D. Blair, R. Padmanabhan, Barry J. Beaty,

Tópico(s)

Insect symbiosis and bacterial influences

Resumo

A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. One infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses.

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