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First Field Trial of an Immunoradiometric Assay for the Detection of Malaria Sporozoites in Mosquitoes

1984; American Society of Tropical Medicine and Hygiene; Volume: 33; Issue: 4 Linguagem: Inglês

10.4269/ajtmh.1984.33.538

1476-1645

Frank H. Collins, Fidel Zavala, Patricia M. Graves, A H Cochrane, Robert W. Gwadz, Jacob A. Akoh, Ruth S. Nussenzweig,

Invertebrate Immune Response Mechanisms

An immunoradiometric assay (IRMA) using a monoclonal antibody to the major surface protein of Plasmodium falciparum sporozoites was used to assess the P. falciparum sporozoite rate in a West African population of Anopheles gambiae (s.l.). Unlike current dissection techniques, the IRMA could detect sporozoite antigen in dried as well as fresh mosquitoes. In a controlled comparison, the sensitivity of the IRMA was comparable that of the dissection technique. Additionally, the IRMA was species specific and quantitative. Sensitivity of the assay was sufficient to detect sporozoite infections resulting from the development of a single oocyst.