Incidence of Grapevine Fanleaf Virus in Iran: A Survey Study and Pro duction of Virus-Free Material Using Meristem Culture and Thermotherapy
2009; Eugen Ulmer Verlag; Volume: 74; Issue: 1 Linguagem: Inglês
ISSN
1611-4434
AutoresS. A. Salami, Ali Ebadi, Zabihollah Zamani, Mina Koohi Habibi,
Tópico(s)Insect-Plant Interactions and Control
ResumoSummary The occurrence, geographical distribution and rate of incidence of Grapevine fanleaf virus (GFLV) were surveyed in 44 vineyards and nurseries in different regions of Iran. GFLV infection was confirmed by mechanical transmission onto herbaceous hosts, DAS-ELISA and IC-RT-PCR. Two thermal conditions, as well as meristem culture were used to produce virus-free material. Results confirmed the presence of GFLV in Bavanat, Shiraz, Jahrom, Maymand, Urmia, Karaj and Naghadeh. Both sanitation protocols significantly reduce the level of GFLV infection, however, a combination of meristem culture and thermotherapy produced 100 % virus-free material from the cultivars ‘Bidaneh Sefid’ and ‘Shahroodi’.
Referência(s)