Radioimmunoassay of gastrin
1970; Elsevier BV; Volume: 58; Issue: 1 Linguagem: Inglês
10.1016/s0016-5085(70)80086-5
ISSN1528-0012
AutoresRosalyn S. Yalow, Solomon A. Berson,
Tópico(s)Neuroendocrine Tumor Research Advances
ResumoA highly specific radioimmunoassay for endogenous human plasma gastrin was developed using guinea pig antisera to crude porcine gastrin.Highly purified 125I-porcine gastrin I, further purified on starch gel electrophoresis after labeling, was employed as tracer, and an anionic exchange resin was used to separate bound and free labeled gastrin.Sensitivity is better than 5 pg per ml.Cholecystokinin-pancreozymin and amino-terminal and carboxyl-terminal gastrin peptides cross-react only poorly in the gastrin-antigastrin system.Plasma gastrin and standard gastrin show parallel inhibition curves against the labeled porcine gastrin.Reproducibility of determinations in different assays is demonstrated.Plasma gastrin is unaffected by boiling for 5 to 10 min.High values of plasma gastrin were observed in 5 patients with Zollinger-Ellison syndrome and in 17 patients with pernicious anemia.Administration of HCl per os or into the stomach in 5 patients with pernicious anemia produced an acute fall in plasma gastrin concentration; the half-time for disappearance of gastrin from plasma was as short as 7 min.There have been several reports on the production of antibodies to gastrin and
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