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Thin-Layer Chromatography Radioassay: A Review

1968; Linguagem: Inglês

10.1007/978-1-4684-7532-6_7

0065-3497

Fred Snyder,

Zeolite Catalysis and Synthesis

Considerable technological progress has been made in the radioassay of analytical and preparative thin-layer chroma­tography (TLC) plates since Mangold's original review of this subject (a chapter in Stahl's book, Dunnschicht-Chromatog­raphic, 62–79, 1962). Although the general approach to radioassay of thin-layer chromatograms is similar to that used in paper chromatography, the two techniques require different radiometric procedures because the physical and chemical nature of the materials used in adsorption TLC are completely different from those encountered in partition separations by paper. Autoradiography, fluorography, strip scanning, zonal scan­ning, and elution procedures are used for radiometric analysis of thin-layer chromatograms. Thin-layer chromatographic profiles of C14 and H3 determined by liquid scintillation zonal scans are superior to other methods in sensitivity and resolu­tion. Recently the entire zonal-scan procedure was com­puterized so that the distribution of the chemical mass or radioactivity along a chromatographic lane can be graphed by an electroplotter directed by the TLC computer program. The program gives a direct printout of area percent, recovery, and counting efficiency. Corroborative TLC (argentation and borate) and gas—liquid chromatography techniques are described for the separation of glyceryl alkyl ethers according to unsaturation, isomeric form, and chain length.