More sensitive test agar for detection of dextranase-producing oral streptococci and identification of two glucan synthesis-defective dextranase mutants of Streptococcus mutans 6715
1979; American Society for Microbiology; Volume: 10; Issue: 6 Linguagem: Inglês
10.1128/jcm.10.6.919-922.1979
ISSN1098-660X
AutoresJacob A. Donkersloot, Robert Harr,
Tópico(s)Oral microbiology and periodontitis research
ResumoA more sensitive test agar was developed to detect oral microbes with relatively low dextranase activity and to identify dextranase-negative mutants. Several oral streptococci that had previously been scored as dextranase negative readily decolorized the new, blue dextran-containing medium. To assess whether dextranase plays a role in glucan synthesis by oral streptococci, various glucan synthesis-defective mutants were tested for dextranase activity on the new medium. Mutants 4 and 27, which do not cause smooth-surface caries and which synthesize more soluble glucan than their parent, Streptococcus mutans 6715-13, were markedly deficient in these dextranase activity tests.
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