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Identification of Protein Interactions by Far Western Analysis

2001; Wiley; Volume: 55; Issue: 1 Linguagem: Inglês

10.1002/0471142727.mb2006s55

1934-3639

Diane G. Edmondson, Sharon Y. Roth,

Advanced Electron Microscopy Techniques and Applications

Far western blotting is a method of identifying protein-protein interactions. One protein of interest is immobilized on a solid support membrane, then probed with a non-antibody protein. Far western blots can be used to identify specific interacting proteins in a complex mixture of proteins. They are particularly useful for examining interactions between proteins that are difficult to analyze by other methods due to solubility problems or because they are difficult to express in cells. This method is performed totally in vitro, and the proteins of interest can be prepared in a variety of ways.