Isolation and Characterization of Four Adenovirus Type 12-Transformed Human Embryo Kidney Cell Lines
1984; Taylor & Francis; Volume: 4; Issue: 1 Linguagem: Inglês
10.1128/mcb.4.1.110-116.1984
ISSN1098-5549
AutoresJoanne L. Whittaker, Philip J. Byrd, Roger J.A. Grand, Phillip H. Gallimore,
Tópico(s)CAR-T cell therapy research
ResumoFour transformed cell lines were established from cultures of human embryo kidney (HEK) cells microinjected or transfected with cloned adenovirus 12 (Ad12) EcoRI-C DNA (0 through 16.5 map units of the left-hand end of the viral genome). Each cell line showed a different growth pattern. Southern blotting demonstrated that all of the cell lines contained Ad12-specific DNA sequences, but in the microinjected isolates these were at a much lower copy number than in the transfected isolate. Two cell lines (Ad12 HEK 1 and 3) appeared to contain tandemly repeated Ad12 EcoRI-C DNA fragments. Immunoprecipitation and Western blotting confirmed that Ad12 early region 1 (E1) proteins were being expressed by all four of the transformed cell lines, but indicated that E1A polypeptide expression was considerably less than E1B polypeptide expression. All of the Ad12-transformed HEK cell lines were tumorigenic when inoculated intracranially into athymic nude mice.
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