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Sp1 Can Displace GHF-1 from Its Distal Binding Site and Stimulate Transcription from the Growth Hormone Gene Promoter

1990; Taylor & Francis; Volume: 10; Issue: 4 Linguagem: Inglês

10.1128/mcb.10.4.1811-1814.1990

1098-5549

Frédéric P. Lemaigre, Dominique A. Lafontaine, Stéphane Courtois, Serge Durviaux, Guy Rousseau,

Pancreatic function and diabetes

DNase I footprinting experiments showed that binding activities of Sp1 and of GHF-1 to its distal site on the human growth hormone gene promoter are mutually exclusive. The kinetics of GHF-1 binding were indicative of positive cooperativity. The Sp1 site did not affect promoter activity in cell-free transcription. Still, Sp1 could compensate partially for the decreased stimulation of transcription seen at low GHF-1 concentrations.