Multicenter evaluation of the Paragon CZETM 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing
1998; American Association for Clinical Chemistry; Volume: 44; Issue: 3 Linguagem: Inglês
10.1093/clinchem/44.3.599
ISSN1530-8561
AutoresJacques Bienvenu, Maria Stella Graziani, François Arpin, H Bernon, Cynthia Blessum, Cristina Marchetti, G Righetti, Manuela Somenzini, Gioacchino Verga, F Aguzzi,
Tópico(s)Innovative Microfluidic and Catalytic Techniques Innovation
ResumoSerum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were < 2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was < 0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations > 10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.
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