Portal de Periódicos da CAPES

miRNA Stability in Frozen Plasma Samples

2015; Multidisciplinary Digital Publishing Institute; Volume: 20; Issue: 10 Linguagem: Inglês

10.3390/molecules201019030

1433-1373

Francesca Balzano, Marta Deiana, Silvia Dei Giudici, Annalisa Oggiano, A Baralla, Sara Pasella, Andrea Mannu, Mario Pescatori, Baingio Porcu, Giuseppe Fanciulli, Angelo Zinellu, Ciriaco Carru, Luca Deiana,

Cancer-related molecular mechanisms research

MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at −80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life.