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Reversed-phase HPLC separation of plasma norepinephrine, epinephrine, and dopamine, with three-electrode coulometric detection.

1989; American Association for Clinical Chemistry; Volume: 35; Issue: 9 Linguagem: Inglês

10.1093/clinchem/35.9.1975

1530-8561

Natale R. Musso, C. Vergassola, Aldo Pende, G Lotti,

Electrochemical sensors and biosensors

We present a method involving minor modifications of previous techniques, in which we use an ion-pair reversed-phase HPLC separation with three-electrode coulometry. Isocratic baseline separations can be obtained in 5 min. The sample preparation procedure, involving extraction with alumina at low temperature, allows good reproducibility (within-run and between-run CVs less than 10%) and improved sensitivity (less than 5 pg of each catecholamine per extract). This method allows approximately 70 low-cost plasma catecholamine analyses to be done in a working day.