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Relevance of Spontaneous Activity to Urinary Bladder Function: An in Vitro and in Vivo Study

1986; Lippincott Williams & Wilkins; Volume: 136; Issue: 2 Linguagem: Inglês

10.1016/s0022-5347(17)44934-2

1527-3792

Robert M. Levin, Michael R. Ruggieri, Satish Velagapudi, David Gordon, Bruce Altman, Alan J. Wein,

Urinary Bladder and Prostate Research

No AccessJournal of Urology1 Aug 1986Relevance of Spontaneous Activity to Urinary Bladder Function: An in Vitro and in Vivo Study Robert M. Levin, Michael R. Ruggieri, Satish Velagapudi, David Gordon, Bruce Altman, and Alan J. Wein Robert M. LevinRobert M. Levin , Michael R. RuggieriMichael R. Ruggieri , Satish VelagapudiSatish Velagapudi , David GordonDavid Gordon , Bruce AltmanBruce Altman , and Alan J. WeinAlan J. Wein View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)44934-2AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail The presence and functional significance (if any) of spontaneous activity in the normal urinary bladder during filling is a controversial subject. One model used by many investigators to study spontaneous activity has been isolated urinary bladder smooth muscle strips. Although spontaneous activity is a property commonly observed in isolated urinary bladder strip preparations, the in vitro whole bladder preparation (rabbit) is devoid of spontaneous activity. Additionally, under normal conditions the in vivo rabbit bladder does not display spontaneous activity during the filling phases of micturition. The present study compares the spontaneous activity of isolated smooth muscle strips, the whole bladder preparation, and the catheterized in vivo bladder (rabbit). The results are as follows: 1) The spontaneous activity (frequency and amplitude) of isolated strips is extremely variable among strips of the same bladder. 2) Spontaneous activity is not affected by the following specific inhibitory compounds: tetrodotoxin, atropine, phentolamine, propranolol and hexamethonium. This indicates that spontaneous activity observed in isolated strips is myogenic in nature and not dependent on the activation of specific autonomic receptors. 3) The in vitro whole bladder preparation shows no spontaneous activity at any volume or pressure unless longitudinal tension is applied. 4) The spontaneous activity of the whole bladder subjected to longitudinal tension is not affected by the same compounds mentioned above. 5) Spontaneous activity of the in vivo bladder is absent at low intravesical volumes and pressures. Spontaneous activity develops upon reaching a critical pressure. However, this activity is completely inhibited by intravenous ganglionic blockade (hexamethonium). In the presence of hexamethonium, the in vivo bladder is devoid of spontaneous activity at any volume or pressure, thus the in vivo "spontaneous activity" is mediated through neuronal reflexes. It is concluded that under normal circumstances the rabbit bladder is devoid of myogenic spontaneous activity and that the spontaneous activity observed in isolated strips is directly related to longitudinal stretch. Since under normal conditions the bladder is not subjected to longitudinal stretch, the spontaneous activity observed in the isolated strip studies has little physiological significance under normal conditions, but could help explain the pathophysiology of certain dysfunctions during the filling stage of micturition. © 1986 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byByrne M, Klausner A, Speich J, Southern J, Habibi J and Ratz P (2013) Fourier Transform Analysis of Rabbit Detrusor Autonomous Contractions Reveals Length Dependent Increases in Tone and Slow Wave Development at Long LengthsJournal of Urology, VOL. 190, NO. 1, (334-340), Online publication date: 1-Jul-2013.Scheepe J, Amelink A, de Jong B, Wolffenbuttel K and Kok D (2011) Changes in Bladder Wall Blood Oxygen Saturation in the Overactive Obstructed BladderJournal of Urology, VOL. 186, NO. 3, (1128-1133), Online publication date: 1-Sep-2011.CAPOLICCHIO G, AITKEN K, GU J, REDDY P and BÄGLI D (2018) EXTRACELLULAR MATRIX GENE RESPONSES IN A NOVEL EX VIVO MODEL OF BLADDER STRETCH INJURYJournal of Urology, VOL. 165, NO. 6 Part 2, (2235-2240), Online publication date: 1-Jun-2001.Levin R, Longhurst P, Kato K, McGuire E, Elbadawi A and Wein A (2018) Comparative Physiology and Pharmacology of the Cat and Rabbit Urinary BladderJournal of Urology, VOL. 143, NO. 4, (848-852), Online publication date: 1-Apr-1990.Kato K, Tong-Long Lin A, Haugaard N, Longhurst P, Wein A and Levin R (2018) Effects of Outlet Obstruction on Glucose Metabolism of the Rabbit Urinary BladderJournal of Urology, VOL. 143, NO. 4, (844-847), Online publication date: 1-Apr-1990. Volume 136Issue 2August 1986Page: 517-521 Advertisement Copyright & Permissions© 1986 by The American Urological Association Education and Research, Inc.Metrics Author Information Robert M. Levin More articles by this author Michael R. Ruggieri More articles by this author Satish Velagapudi More articles by this author David Gordon More articles by this author Bruce Altman More articles by this author Alan J. Wein More articles by this author Expand All Advertisement PDF downloadLoading ...