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RelB, a new Rel family transcription activator that can interact with p50-NF-kappa B.

1992; Taylor & Francis; Volume: 12; Issue: 2 Linguagem: Inglês

10.1128/mcb.12.2.674

1098-5549

Rolf-Peter Ryseck, Paulina Bull, Mónica Takamiya, Vincent Bours, Ulrich Siebenlist, Paweł Dobrzański, R Bravo,

NF-κB Signaling Pathways

We have identified a serum-inducible gene, relB, which encodes a protein of 558 amino acids containing a region with high similarity to c-Rel and other members of the Rel family. Transcriptional activation analysis of GAL4-RelB fusion proteins in yeast cells reveals that RelB contains in its C-terminal 180 amino acids a transcriptional activation domain. The N-terminal part including the region of similarity with the Rel family shows no detectable transcriptional activity. RelB does not bind with high affinity to NF-kappa B sites, but heterodimers between RelB and p50-NF-kappa B do bind to different NF-kappa B-binding sites with a similar affinity to that shown by p50-NF-kappa B homodimers. However, RelB/p50-NF-kappa B heterodimers, in contrast to p50-NF-kappa B homodimers, transactivate transcription of a promoter containing a kappa B-binding site.