Cytochalasin D alters the rate of synthesis of some HEp-2 cytoskeletal proteins. Examination by two-dimensional gel electrophoresis
1986; Wiley; Volume: 155; Issue: 3 Linguagem: Inglês
10.1111/j.1432-1033.1986.tb09521.x
ISSN1432-1033
Autores Tópico(s)Skin and Cellular Biology Research
Resumo1. The most abundant proteins of HEp‐2 cells were resolved by two‐dimensional gel electrophoresis. The protein spots corresponding to several cytoskeletal proteins (vimentin, α‐tubulin, β‐tubulin, α‐actinin, tropomyosins, and cytokeratins) were identified by comigration with protein markers or by immunological techniques. 2. After treatment of HEp‐2 cells with 0.2 μM or 2.0 μM cytochalasin D for 20 h, radioautograms of two‐dimensional gel patterns of lysates from cells pulse‐labeled with [ 35 S]methionine indicated that the drug altered the rate of synthesis of some proteins. The relative rate of synthesis of the identified cytoskeletal proteins was measured. Synthesis of α‐actinin, the higher‐molecular‐mass pair of tropomyosins and actin were similarly increased with cytochalasin D treatment, suggesting coordinate induction. Vimentin and tubulin synthesis was depressed. One cytokeratin exhibited an increase in synthesis comparable to actin, another was increased to a lesser extent and one was decreased.
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