Posterior talar process as a suitable cell source for treatment of cartilage and osteochondral defects of the talus
2015; Wiley; Volume: 11; Issue: 7 Linguagem: Inglês
10.1002/term.2092
ISSN1932-7005
AutoresSusana Correia, Joana Silva‐Correia, Hélder Pereira, Raphaël F. Canadas, Alain da Silva Morais, A. M. Frias, Rui A. Sousa, C. Niek van Dijk, João Espregueira‐Mendes, Rui L. Reis, Joaquím M. Oliveira,
Tópico(s)Wound Healing and Treatments
ResumoJournal of Tissue Engineering and Regenerative MedicineVolume 11, Issue 7 p. 1949-1962 Research Article Posterior talar process as a suitable cell source for treatment of cartilage and osteochondral defects of the talus S. I. Correia, S. I. Correia 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorJ. Silva-Correia, J. Silva-Correia 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorH. Pereira, H. Pereira 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, Portugal Clínica do Dragão - Espregueira-Mendes Sports Centre - FIFA Medical Centre of Excellence, F.C. Porto Stadium, Minho University and Porto University Research Centre, Portugal Orthopaedic Department Centro Hospitalar Póvoa de Varzim, Vila do Conde, PortugalSearch for more papers by this authorR. F. Canadas, R. F. Canadas 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorA. da Silva Morais, A. da Silva Morais 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorA. M. Frias, A. M. Frias 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal Stemmatters, Biotecnologia e Medicina Regenerativa SA, 4805-017 Guimarães, PortugalSearch for more papers by this authorR. A. Sousa, R. A. Sousa 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal Stemmatters, Biotecnologia e Medicina Regenerativa SA, 4805-017 Guimarães, PortugalSearch for more papers by this authorC. N. van Dijk, C. N. van Dijk Clínica do Dragão - Espregueira-Mendes Sports Centre - FIFA Medical Centre of Excellence, F.C. Porto Stadium, Minho University and Porto University Research Centre, Portugal Orthopaedic Department, Amsterdam Medical Centre, The NetherlandsSearch for more papers by this authorJ. Espregueira-Mendes, J. Espregueira-Mendes 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, Portugal Clínica do Dragão - Espregueira-Mendes Sports Centre - FIFA Medical Centre of Excellence, F.C. Porto Stadium, Minho University and Porto University Research Centre, PortugalSearch for more papers by this authorR. L. Reis, R. L. Reis 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorJ. M. Oliveira, Corresponding Author J. M. Oliveira miguel.oliveira@dep.uminho.pt 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalCorrespondence to: Joaquim M. Oliveira, 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Portugal. E-mail: miguel.oliveira@dep.uminho.ptSearch for more papers by this author S. I. Correia, S. I. Correia 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorJ. Silva-Correia, J. Silva-Correia 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorH. Pereira, H. Pereira 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, Portugal Clínica do Dragão - Espregueira-Mendes Sports Centre - FIFA Medical Centre of Excellence, F.C. Porto Stadium, Minho University and Porto University Research Centre, Portugal Orthopaedic Department Centro Hospitalar Póvoa de Varzim, Vila do Conde, PortugalSearch for more papers by this authorR. F. Canadas, R. F. Canadas 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorA. da Silva Morais, A. da Silva Morais 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorA. M. Frias, A. M. Frias 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal Stemmatters, Biotecnologia e Medicina Regenerativa SA, 4805-017 Guimarães, PortugalSearch for more papers by this authorR. A. Sousa, R. A. Sousa 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal Stemmatters, Biotecnologia e Medicina Regenerativa SA, 4805-017 Guimarães, PortugalSearch for more papers by this authorC. N. van Dijk, C. N. van Dijk Clínica do Dragão - Espregueira-Mendes Sports Centre - FIFA Medical Centre of Excellence, F.C. Porto Stadium, Minho University and Porto University Research Centre, Portugal Orthopaedic Department, Amsterdam Medical Centre, The NetherlandsSearch for more papers by this authorJ. Espregueira-Mendes, J. Espregueira-Mendes 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, Portugal Clínica do Dragão - Espregueira-Mendes Sports Centre - FIFA Medical Centre of Excellence, F.C. Porto Stadium, Minho University and Porto University Research Centre, PortugalSearch for more papers by this authorR. L. Reis, R. L. Reis 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalSearch for more papers by this authorJ. M. Oliveira, Corresponding Author J. M. Oliveira miguel.oliveira@dep.uminho.pt 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal ICVS/3Bs, PT Government Associate Laboratory, Braga/Guimarães, PortugalCorrespondence to: Joaquim M. Oliveira, 3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Portugal. E-mail: miguel.oliveira@dep.uminho.ptSearch for more papers by this author First published: 29 October 2015 https://doi.org/10.1002/term.2092Citations: 5Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues and investigate their potential as a new source of viable cells for application in tissue engineering and regenerative medicine. SP and OT tissues obtained from six patients were characterized by micro-computed tomography and histological, histomorphometric and immunohistochemical analyses. Proliferation and viability of isolated cells were evaluated by MTS assay, DNA quantification and live/dead staining. The TUNEL assay was performed to evaluate cell death by apoptosis. Moreover, the production of extracellular matrix was evaluated by toluidine blue staining, whereas cells phenotype was investigated by flow cytometry. Characterization of ankle explants showed the presence of a cartilage tissue layer in both SP and OT tissues, which represented at least 20%, on average, of the explant. The presence of type II collagen was detected in the extracellular matrix. Isolated cells presented a round morphology typical of chondrocytes. In in vitro studies, cells were viable and proliferating for up to 21 days of culture. No signs of apoptosis were detected. Flow-cytometry analysis revealed that isolated cells maintained the expression of several chondrocytic markers during culture. The results indicated that the SP and OT tissues were a reliable source of viable chondrocytes, which could find promising applications in ACI/MACI strategies with minimal concerns regarding donor zone complications. Copyright © 2015 John Wiley & Sons, Ltd. Citing Literature Supporting Information Figure S1. Scheme of the human ankle tissues, identifying the location of Stieda's process, the os trigonum (OT) and SP after arthroscopic excision Figure S2. Fluorescence microscopy images of Stieda's process (SP1) after immunohistochemical localization of type I collagen, control sample without staining or antibodies and sample treated with type I collagen primary antibody and AlexaFluor 488 secondary antibody Figure S3. Cartilage cell counting within each sample Table S1. Primary and fluorescent-labelled secondary antibodies used in immunohistochemical analysis Supplementary Figures Captions Filename Description term2092-sup-0001-SI.tifTIFF image, 3.6 MB Supporting info item term2092-sup-0002-SI.tifTIFF image, 2.2 MB Supporting info item term2092-sup-0003-SI.tifTIFF image, 1.2 MB Supporting info item term2092-sup-0004-SI.docWord document, 11.1 KB Supporting info item term2092-sup-0005-SI.docxWord document, 14.5 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume11, Issue7July 2017Pages 1949-1962 RelatedInformation
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