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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Experimental design of a liposomal lipid system: A potential strategy for paclitaxel-based breast cancer treatment

2015; Elsevier BV; Volume: 136; Linguagem: Inglês

10.1016/j.colsurfb.2015.09.055

ISSN

1873-4367

Autores

Marcos Vinícius Barbosa, Liziane O.F. Monteiro, Guilherme Carneiro, Andréa R. Malagutti, José Mário Carneiro Vilela, Margareth Spangler Andrade, Mônica Cristina Oliveira, Álvaro Dutra de Carvalho Júnior, Elaine Amaral Leite,

Tópico(s)

Lipid Membrane Structure and Behavior

Resumo

Paclitaxel (PTX) is widely used as a first-line treatment for patients with metastatic breast cancer; however, its poor water solubility represents a major challenge for parenteral administration. The encapsulation of the PTX in drug-delivery systems with high affinity for tumor sites could improve the uptake and increase its therapeutic efficacy. In this work, long-circulating and pH-sensitive PEG-coated (SpHL-PTX) and PEG-folate-coated liposomes containing PTX (SpHL-FT-PTX) were prepared, and the physicochemical properties and in vitro cytotoxic activity were evaluated. Both formulations presented adequate physicochemical properties, including a mean diameter smaller than 200 nm, zeta potential values near the neutral range, and an encapsulation percentage higher than 93%. Moreover, SpHL-FT-PTX showed a good stability after storage for 100 days at 4 °C. The viability studies on breast cancer cell lines (MDA-MB-231 and MCF-7) demonstrated cytotoxic activity more pronounced for SpHL-FT-PTX than for SpHL-PTX or free drug for both tumor cell lines. This activity was reduced to a rate comparable to SpHL-PTX when the cells were previously treated with folic acid in order to saturate the receptors. In contrast, in the normal cell line (L929), cell viability was decreased only by free or liposomal PTX in the highest concentrations. A significantly higher selectivity index was obtained after SpHL-FT-PTX treatment compared to SpHL-PTX and free PTX. Therefore, the results of the present work suggest that SpHL-FT-PTX can be a promising formulation for the treatment of metastatic breast cancer.

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