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Effect of Topical Ginkgo biloba Extract on Steroid-Induced Changes in the Trabecular Meshwork and Intraocular Pressure

2008; American Medical Association; Volume: 126; Issue: 12 Linguagem: Inglês

10.1001/archophthalmol.2008.512

1538-3601

Liyun Jia,

Cerebrovascular and genetic disorders

Objective: To study the effects of Ginkgo biloba extract (GBE) on dexamethasone (DEX)-induced ocular hypertension.Methods: Rabbits aged 7 weeks received topical Tobra-DEX (Alcon Labs, Hünenberg, Switzerland) and/or 5 µg of GBE four times daily for 14 days.Intraocular pressure (IOP) was recorded every 3 days.After enucleation, trabecular meshwork (TM) cellularity and extracellular matrix deposition were graded.The effect of GBE on apoptosis and expression of myocilin and cell stressrelated genes in DEX-treated human TM cells were studied by immunofluorescence, Western blotting, and quantitative polymerase chain reaction.Results: Ginkgo biloba extract suppressed DEXinduced IOP elevation in rabbits.It reduced the DEXassociated accumulation of extracellular materials within the cribriform layers of the TM and achieved better TM cellularity.In cultured human TM cells, GBE substantially attenuated anti-Fas ligand-induced apoptosis and reduced DEX-induced myocilin expression.Ginkgo biloba extract modulated the expression of ␣B-crystallin and heat-shock proteins 70 and 90␣ but not other stressrelated genes.Furthermore, changes associated with DEX were found less in GBE-treated or GBE-primed TM cells. Conclusion:We showed that GBE, a nontoxic, antiapoptotic, herbal compound significantly suppressed steroid-induced IOP elevation in rabbits and it seems to prevent the adverse effects of DEX on TM cells.