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'Lipoteichoic acid' of Bifidobacterium bifidum subspecies pennsylvanicum DSM 20239. A lipoglycan with monoglycerophosphate side chains

1987; Wiley; Volume: 165; Issue: 3 Linguagem: Inglês

10.1111/j.1432-1033.1987.tb11488.x

1432-1033

Werner Fischer,

Probiotics and Fermented Foods

The lipid macroamphiphile of Bifidobacterium bifidum subsp. pennsylvanicum DSM 20239 was extracted with phenol/water and purified by treatment with nucleases and hydrophobic interaction chromatography. From analytical data, the results of Smith degradation, hydrolysis with HF and methylation studies, the following structure is proposed: where n and m are approximately 7–10 and 8–15, respectively. The monoglycerophosphate residues have the sn-glycero-1-phosphate configuration; 20–50% of them are substituted with L-alanine in ester linkage. The lipid anchor is most likely a galactosyldiacylglycerol, part of which carries a third fatty acid. This is the first example among gram-positive bacteria of a glycerophosphate-containing lipid macro-amphiphile that carries the glycerophosphate residues as monomeric side chains on a lipoglycan. Further, it contains l-alanine in place of the d-alanine found in lipoteichoic acids.