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Oxidation of Manganese and Iron by Leptothrix discophora : Use of N,N,N′,N′ -Tetramethyl- p -Phenylenediamine as an Indicator of Metal Oxidation

1990; American Society for Microbiology; Volume: 56; Issue: 11 Linguagem: Inglês

10.1128/aem.56.11.3458-3462.1990

1098-5336

Elisabeth W. de Vrind‐de Jong, Paul L. A. M. Corstjens, Egbert S. Kempers, Peter Westbroek, Johannes P. M. de Vrind,

Enzyme-mediated dye degradation

A new method for the quantification and characterization of manganese-oxidizing activity by spent culture medium of Leptothrix discophora SS-1 was developed. It is based on the formation of the dye Wurster blue from N,N,N′,N′ -Tetramethyl- p -phenylenediamine by oxidized manganese generated in the spent medium. The kinetic parameters thus obtained agreed well with data obtained with other methods. It was also possible to demonstrate iron oxidation by spent culture medium. The kinetics of the process and inhibition by enzyme poisons suggest that iron oxidation is enzymatically catalyzed. Probably two different factors are involved in manganese and iron oxidation.