Portal de Periódicos da CAPES

RNA-SEQ: A GLANCE AT TECHNOLOGIES AND METHODOLOGIES

2014; National University of Colombia; Volume: 20; Issue: 2 Linguagem: Inglês

10.15446/abc.v20n2.43639

1900-1649

Seyed Mehdi Jazayeri, Luz Marina Melgarejo, Hernán Mauricio Romero,

Molecular Biology Techniques and Applications

RNA Sequencing (RNA-Seq) is a newly born tool that has revolutionized the post-genomic era. The data produced by RNA-Seq, sequencing technologies and use of bioinformatics are exploding rapidly. In recent years, RNA-Seq has been the method of choice for profiling dynamic transcriptome taking advantage of high throughput sequencing technologies. RNA-Seq studies have shown the transcriptome magnitude, notion and complexity. From 2008, as its introduction year, the relevant reports on RNA-Seq have been multiplied by more than 2822 times just in 6 years. RNA-Seq also contributes a more accurate gene expression and transcript isoform estimation than other methods. Furthermore, some of the potential applications for RNA-Seq cannot be conducted by other methods and as yet are unique to RNA-Seq. As RNA-Seq approaches increase in speed and decrease in cost, more distinct researches are applied and become more common and accurate. RNA-Seq is a cross and interdisciplinary method that interconnects biology to other scientific topics. This article describes RNA-Seq approach, technologies, methodologies, implementation and methods done so far in characterizing and profiling transcriptomes. RESUMEN En los ultimos anos, la tecnica RNA-Seq ha tenido un desarrollo acelerado y se ha convertido en el metodo de eleccion para el estudio y la caracterizacion de los transcriptomas dinamicos, aprovechando las tecnologias de secuenciacion de alto rendimiento. Estudios aplicando RNA-Seq han mostrado la magnitud, nocion y complejidad del transcripotma. A partir de 2008, ano de introduccion de la tecnica, los estudios con RNA-Seq se han multiplicados por mas de 2822 veces solo en 6 anos. Al compararse con otros metodos, los estudios empleando RNA-Seq contribuyen a una estimacion mas precisa de la expresion genica y de las isoformas de los transcriptos. Ademas, algunas de las aplicaciones potenciales de RNA-Seq no se pueden llevar a cabo con otros metodos. El uso de RNA-Seq aumenta la velocidad de obtencion de informacion y disminuye los costos, logrando con su uso, que investigaciones diversas se vuelvan mas frecuentes y precisas. RNA-Seq es un metodo interdisciplinario que interconecta la biologia a otros temas cientificos. En este articulo se describe el planteamiento de la tecnologia RNA-Seq, metodologias y los metodos realizados en la caracterizacion de transcriptomas.