Portal de Periódicos da CAPES

Use of microsatellite DNA and amplified fragment length polymorphism for Cherry salmon (Oncorhynchus masou) complex identification

2010; Wiley; Volume: 41; Issue: 9 Linguagem: Inglês

10.1111/j.1365-2109.2010.02533.x

1365-2109

Te‐Hua Hsu, Zhiyong Wang, Keisuke Takata, Hiroshi Onozato, Takuya Hara, Jin‐Chywan Gwo,

Fish Ecology and Management Studies

Aquaculture ResearchVolume 41, Issue 9 p. e316-e325 Use of microsatellite DNA and amplified fragment length polymorphism for Cherry salmon (Oncorhynchus masou) complex identification Te-Hua Hsu, Te-Hua Hsu Department of Aquaculture, Taiwan National Ocean University, Keelung, TaiwanSearch for more papers by this authorZhi-Yong Wang, Zhi-Yong Wang Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorKeisuke Takata, Keisuke Takata Department of Biology, Shinshu University, Matsumoto, Nagano, JapanSearch for more papers by this authorHiroshi Onozato, Hiroshi Onozato Matsumoto Institute of Microorganisms, Matsumoto, Nagano, JapanSearch for more papers by this authorTakuya Hara, Takuya Hara Department of Ocean Science, Tokyo University of Marine Science and Technology, Tokyo, JapanSearch for more papers by this authorJin-Chywan Gwo, Jin-Chywan Gwo Department of Aquaculture, Taiwan National Ocean University, Keelung, TaiwanSearch for more papers by this author Te-Hua Hsu, Te-Hua Hsu Department of Aquaculture, Taiwan National Ocean University, Keelung, TaiwanSearch for more papers by this authorZhi-Yong Wang, Zhi-Yong Wang Fisheries College, Jimei University, Xiamen, ChinaSearch for more papers by this authorKeisuke Takata, Keisuke Takata Department of Biology, Shinshu University, Matsumoto, Nagano, JapanSearch for more papers by this authorHiroshi Onozato, Hiroshi Onozato Matsumoto Institute of Microorganisms, Matsumoto, Nagano, JapanSearch for more papers by this authorTakuya Hara, Takuya Hara Department of Ocean Science, Tokyo University of Marine Science and Technology, Tokyo, JapanSearch for more papers by this authorJin-Chywan Gwo, Jin-Chywan Gwo Department of Aquaculture, Taiwan National Ocean University, Keelung, TaiwanSearch for more papers by this author First published: 19 August 2010 https://doi.org/10.1111/j.1365-2109.2010.02533.xCitations: 12 Correspondence: J-C Gwo, Department of Aquaculture, Taiwan National Ocean University, No. 2 Pei-Ning Road, Keelung 20224, Taiwan. E-mail: gwojc@hotmail.com Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Formosa landlocked salmon (Oncorhynchus masou formosanus), an endemic, critically endangered subspecies of Cherry salmon (Oncorhynchus masou) complex, is only found in Taiwan. Because the eyed eggs and ungutted carcasses of Pacific salmons (genus Oncorhynchus) are imported for aquaculture and food to Taiwan from overseas every year, the requirement for preventing illegal trade or accidental commercial imports to avoid unwanted fish from contaminating the gene pool of Formosa landlocked salmon and infect them with diseases is critical for the conservation of Formosa landlocked salmon. Traditional morphology-based species identification is impossible for salmon eggs and larvae that lack clearly defined morphological features. In the present study, the genetic differences among four subspecies (Oncorhynchus masou ishikawae, O. masou subsp., Oncorhynchus masou masou and O. masou formosanus) of Cherry salmon complex were determined from microsatellite DNA and amplified fragment length polymorphism analyses. We successfully generated a genetic marker to aid traditional taxonomy and investigate the integrity of the current taxonomic status among members of Cherry salmon complex. Use of molecular markers, in combination with traditional morphological identification, is a promising tool for identifying four closely related subspecies of Cherry salmon complex. Citing Literature Volume41, Issue9August 2010Pages e316-e325 RelatedInformation