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The subpopulation of microglia sensitive to neurotransmitters/neurohormones is modulated by stimulation with LPS, interferon-γ, and IL-4

2014; Wiley; Volume: 62; Issue: 5 Linguagem: Inglês

10.1002/glia.22633

1098-1136

Maria Pannell, Frank Szulzewsky, Vitali Matyash, Susanne A. Wolf, Helmut Kettenmann,

Adenosine and Purinergic Signaling

GliaVolume 62, Issue 5 p. 667-679 Original Article The subpopulation of microglia sensitive to neurotransmitters/neurohormones is modulated by stimulation with LPS, interferon-γ, and IL-4 Maria Pannell, Maria Pannell Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorFrank Szulzewsky, Frank Szulzewsky Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorVitali Matyash, Vitali Matyash Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorSusanne A. Wolf, Susanne A. Wolf Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorHelmut Kettenmann, Corresponding Author Helmut Kettenmann Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanyAddress correspondence to Helmut Kettenmann, Cellular Neurosciences, Max Delbrück Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125 Berlin, Germany. E-mail: kettenmann@mdc-berlin.deSearch for more papers by this author Maria Pannell, Maria Pannell Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorFrank Szulzewsky, Frank Szulzewsky Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorVitali Matyash, Vitali Matyash Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorSusanne A. Wolf, Susanne A. Wolf Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanySearch for more papers by this authorHelmut Kettenmann, Corresponding Author Helmut Kettenmann Max Delbrück Center for Molecular Medicine, Berlin-Buch, GermanyAddress correspondence to Helmut Kettenmann, Cellular Neurosciences, Max Delbrück Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125 Berlin, Germany. E-mail: kettenmann@mdc-berlin.deSearch for more papers by this author First published: 07 February 2014 https://doi.org/10.1002/glia.22633Citations: 51Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Recently, neurotransmitters/neurohormones have been identified as factors controlling the function of microglia, the immune competent cells of the central nervous system. In this study, we compared the responsiveness of microglia to neurotransmitters/neurohormones. We freshly isolated microglia from healthy adult C57Bl/6 mice and found that only a small fraction (1–20%) responded to the application of endothelin, histamine, substance P, serotonin, galanin, somatostatin, angiotensin II, vasopressin, neurotensin, dopamine, or nicotine. In cultured microglia from neonatal and adult mice, a similarly small population of cells responded to these neurotransmitters/neurohormones. To induce a proinflammatory phenotype, we applied lipopolysaccaride (LPS) or interferon-gamma (IFN-γ) to the cultures for 24 h. Several of the responding populations increased; however, there was no uniform pattern when comparing adult with neonatal microglia or LPS with IFN-γ treatment. IL-4 as an anti-inflammatory substance increased the histamine-, substance P-, and somatostatin-sensitive populations only in microglia from adult, but not in neonatal cells. We also found that the expression of different receptors was not strongly correlated, indicating that there are many different populations of microglia with a distinct set of receptors. Our results demonstrate that microglial cells are a heterogeneous population with respect to their sensitivity to neurotransmitters/neurohormones and that they are more responsive in defined activation states. GLIA 2014;62:667–679 Citing Literature Volume62, Issue5May 2014Pages 667-679 RelatedInformation