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Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine

1989; American Society for Microbiology; Volume: 63; Issue: 3 Linguagem: Inglês

10.1128/jvi.63.3.1338-1344.1989

1098-5514

Gareth D. Westrop, K A Wareham, D. M. A. Evans, G. Dunn, Philip D. Minor, D. I. Magrath, F. Taffs, S.A. Marsden, Michael A. Skinner, G. C. Schild,

Virology and Viral Diseases

The poliovirus type 3 Sabin oral poliovirus vaccine strain P3/Leon/12a1b differs in nucleotide sequence from its neurovirulent progenitor P3/Leon/37 by just 10 point mutations. The contribution of each mutation to the attenuation phenotype of the vaccine strain was determined by the construction of a series of recombinant viruses from infectious cDNA clones. The neurovirulence testing of recombinant viruses indicated that the attenuation phenotype is determined by just two point mutations: a C to U in the noncoding region at position 472 and a C to U at nucleotide 2034 which results in a serine-to-phenylalanine amino acid substitution in the structural protein VP3.