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Glutamate Dehydrogenase Is Not Essential for Glutamate Formation by Corynebacterium glutamicum

1993; American Society for Microbiology; Volume: 59; Issue: 7 Linguagem: Inglês

10.1128/aem.59.7.2329-2331.1993

1098-5336

Elke R. Börmann-El Kholy, Bernhard J. Eikmanns, Marcella Gutmann, Hermann Sahm,

Amino Acid Enzymes and Metabolism

Two Corynebacterium glutamicum strains, one being glutamate dehydrogenase (GDH) negative and the other possessing 11-fold-higher specific GDH activity than the parental wild type, were constructed and used to analyze the role of GDH in C. glutamicum . The results indicate (i) that GDH is dispensable for glutamate synthesis required for growth and (ii) that although a high level of GDH increases the intracellular glutamate pool, the level of GDH has no influence on glutamate secretion.