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New CYP17 Hydroxylase Inhibitors: Synthesis, Biological Evaluation, QSAR, and Molecular Docking Study of New Pregnenolone Analogs

2014; Wiley; Volume: 347; Issue: 12 Linguagem: Inglês

10.1002/ardp.201400255

1521-4184

Najim A. Al‐Masoudi, Dawood S. Ali, Bahjat A. Saeed, Rolf W. Hartmann, Matthias Engel, Sajid Rashid, Aamer Saeed,

Chemical Reactions and Isotopes

A new series of pregnenonlone analogs were synthesized and evaluated for their inhibitory activity against cytochrome P450 (CYP17 hydroxylase enzyme). In general, the 5‐aryl‐1,3,4‐thiadiazol‐2‐yl)‐imino‐pregnenolone derivatives 11 – 15 were more active than the sulfonate 24 – 31 and the ester 37 – 41 analogs. Derivative 12 showed optimal activity in this series, with IC 50 values of 2.5 µM compared with the standard abiraterone (IC 50 = 0.07 µM). However, the analogs 11 and 25 showed a better selectivity profile (81.5 and 82.7% inhibition of hydroxylase, respectively), which may be a useful lead in CYP17 inhibition studies. Molecular docking studies demonstrated quite similar binding patterns of all new pregnenolone derivatives at the active site of CYP17 through hydrogen bonding and hydrophobic interaction.