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B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties.

1988; Springer Nature; Volume: 7; Issue: 11 Linguagem: Inglês

10.1002/j.1460-2075.1988.tb03220.x

1460-2075

Nobuo Sakaguchi, Shin-ichiro Kashiwamura, Masao Kimoto, Philipp Thalmann, Fritz Melchers,

Immunodeficiency and Autoimmune Disorders

Research Article1 November 1988free access B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties. N. Sakaguchi N. Sakaguchi Department of Immunology, Saga Medical School, Japan. Search for more papers by this author S. Kashiwamura S. Kashiwamura Department of Immunology, Saga Medical School, Japan. Search for more papers by this author M. Kimoto M. Kimoto Department of Immunology, Saga Medical School, Japan. Search for more papers by this author P. Thalmann P. Thalmann Department of Immunology, Saga Medical School, Japan. Search for more papers by this author F. Melchers F. Melchers Department of Immunology, Saga Medical School, Japan. Search for more papers by this author N. Sakaguchi N. Sakaguchi Department of Immunology, Saga Medical School, Japan. Search for more papers by this author S. Kashiwamura S. Kashiwamura Department of Immunology, Saga Medical School, Japan. Search for more papers by this author M. Kimoto M. Kimoto Department of Immunology, Saga Medical School, Japan. Search for more papers by this author P. Thalmann P. Thalmann Department of Immunology, Saga Medical School, Japan. Search for more papers by this author F. Melchers F. Melchers Department of Immunology, Saga Medical School, Japan. Search for more papers by this author Author Information N. Sakaguchi1, S. Kashiwamura1, M. Kimoto1, P. Thalmann1 and F. Melchers1 1Department of Immunology, Saga Medical School, Japan. The EMBO Journal (1988)7:3457-3464https://doi.org/10.1002/j.1460-2075.1988.tb03220.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A gene, called m-mb-1, was isolated from a murine pre-B-minus T lymphocyte subtracted library. It was found expressed as mRNA at low to medium abundance in early progenitors of the B lineage, in pre-B and mature B lineage cell lines, in normal resting B lymphocytes and in polyclonally activated B cell blasts. The gene was not expressed in plasmacytomas, in cell lines of the monocyte/macrophage, the T lymphocyte or the fibroblast lineages, nor in thymus, liver, heart, kidney, lung or brain. The nucleotide sequence of the m-mb-1 gene encodes a putative membrane glycoprotein with 220 amino acids, which includes a leader sequence, a putative extracellular domain with two potential N-glycosylation sites, a transmembrane portion and a putative intracellular domain. The partial sequence of a human homologue, h-mb-1, shows nearly 90% homology in nucleotide as well as amino acid sequences to the murine form of a stretch of the putative intracytoplasmic domain. Antibodies raised against a fusion protein of m-mb-1 with protein A, affinity purified for their m-mb-1 specificity, stained pre-B and mature B cell lines on their surface, but did not stain T cell lines and fibroblasts. Antibodies raised against a stretch of 20 amino acids of the putative intracellular domain with 90% homology between the mouse and human protein did not stain the surface of any cell lines tested.(ABSTRACT TRUNCATED AT 250 WORDS) Previous ArticleNext Article Volume 7Issue 111 November 1988In this issue RelatedDetailsLoading ...