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BIBTEX RIS

Fecal short-chain fatty acid (SCFA) analysis by capillary gas-liquid chromatography

1987; Elsevier BV; Volume: 46; Issue: 4 Linguagem: Inglês

10.1093/ajcn/46.4.641

ISSN

1938-3207

Autores

W Scheppach, C. Fabian, H. Kasper,

Tópico(s)

Microscopic Colitis

Resumo

A new method is described for short-chain fatty acid (SCFA) analysis in stool samples. After sample purification by acid vacuum transfer and concentration by alkaline freeze-drying, SCFAs were measured by gas-liquid chromatography using a capillary column. Peak resolution and reproducibility were superior to SCFA analysis on a conventional glass column. With this new technique SCFA were quantitated in stool samples of six healthy volunteers on a basal diet with and without a fermentable dietary fiber preparation; fiber had no effect on fecal SCFA concentration. In two patients with a short-bowel syndrome, SCFA could be demonstrated in stool samples, indicating active fermentation.

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