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A screen of low-copy nuclear genes reveals the LFY gene as phylogenetically informative in closely related species of orchids ( Ophrys )

2007; Wiley; Volume: 56; Issue: 2 Linguagem: Inglês

10.1002/tax.562019

1996-8175

Philipp M. Schlüter, Gudrun Köhl, Tod F. Stuessy, Hannes F. Paulus,

Plant and animal studies

This paper presents PCR primers and PCR conditions for low‐copy nuclear genes in Ophrys and related orchid genera identified via screening of both published and newly designed primers. For Ophrys , the most useful markers identified in this screen are the LFY / FLO gene which contains an intron of 2 kb size and the MADS‐box PI / GLO gene whose 2 first introns contain single nucleotide polymorphisms with variation at the populational level. In the taxa tested, our PCR primers amplified single‐copy regions. Phylogenetic analysis of closely related taxa of Ophrys section Pseudophrys , based on LFY , revealed the following groups that are delimited by morphology: O. lutea s.l.; O. omegaifera s.l. with O. iricolor nested in this group; the two O. fusca s.l. taxa, O. leucadica and O. bilunulata ; and the O. fusca s.l. taxon O. cinereophila together with a group of endemics from Crete.