Properties of dopaminergic neurons in organotypic mesencephalic-striatal co-cultures - evidence for a facilitatory effect of dopamine on the glutamatergic input mediated by α-1 adrenergic receptors
2011; Wiley; Volume: 33; Issue: 9 Linguagem: Inglês
10.1111/j.1460-9568.2011.07659.x
ISSN1460-9568
AutoresMaria Letizia Cucchiaroni, Peter S. Freestone, Nicola Berretta, Maria Teresa Viscomi, Elisa Bisicchia, Hideyuki Okano, Marco Molinari, Giorgio Bernardi, Janusz Lipski, Nicola Biagio Mercuri, Ezia Guatteo,
Tópico(s)Receptor Mechanisms and Signaling
ResumoEuropean Journal of NeuroscienceVolume 33, Issue 9 p. 1622-1636 Properties of dopaminergic neurons in organotypic mesencephalic–striatal co-cultures – evidence for a facilitatory effect of dopamine on the glutamatergic input mediated by α-1 adrenergic receptors Maria L. Cucchiaroni, Maria L. Cucchiaroni Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy M. L. C. and P.S.F. contributed equally to this study.Search for more papers by this authorPeter S. Freestone, Peter S. Freestone Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand M. L. C. and P.S.F. contributed equally to this study.Search for more papers by this authorNicola Berretta, Nicola Berretta Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this authorMaria T. Viscomi, Maria T. Viscomi Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this authorElisa Bisicchia, Elisa Bisicchia Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Biomedical Sciences, University of Teramo, Teramo, ItalySearch for more papers by this authorHideyuki Okano, Hideyuki Okano Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, JapanSearch for more papers by this authorMarco Molinari, Marco Molinari Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this authorGiorgio Bernardi, Giorgio Bernardi Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Neuroscience, University of Rome Tor Vergata, Rome, ItalySearch for more papers by this authorJanusz Lipski, Janusz Lipski Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New ZealandSearch for more papers by this authorNicola B. Mercuri, Nicola B. Mercuri Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Neuroscience, University of Rome Tor Vergata, Rome, ItalySearch for more papers by this authorEzia Guatteo, Ezia Guatteo Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this author Maria L. Cucchiaroni, Maria L. Cucchiaroni Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy M. L. C. and P.S.F. contributed equally to this study.Search for more papers by this authorPeter S. Freestone, Peter S. Freestone Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand M. L. C. and P.S.F. contributed equally to this study.Search for more papers by this authorNicola Berretta, Nicola Berretta Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this authorMaria T. Viscomi, Maria T. Viscomi Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this authorElisa Bisicchia, Elisa Bisicchia Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Biomedical Sciences, University of Teramo, Teramo, ItalySearch for more papers by this authorHideyuki Okano, Hideyuki Okano Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, JapanSearch for more papers by this authorMarco Molinari, Marco Molinari Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this authorGiorgio Bernardi, Giorgio Bernardi Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Neuroscience, University of Rome Tor Vergata, Rome, ItalySearch for more papers by this authorJanusz Lipski, Janusz Lipski Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New ZealandSearch for more papers by this authorNicola B. Mercuri, Nicola B. Mercuri Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy Department of Neuroscience, University of Rome Tor Vergata, Rome, ItalySearch for more papers by this authorEzia Guatteo, Ezia Guatteo Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, ItalySearch for more papers by this author First published: 31 March 2011 https://doi.org/10.1111/j.1460-9568.2011.07659.xCitations: 8 Dr E. Guatteo, as above.E-mail: e.guatteo@hsantalucia.it Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Organotypic cultures (OCs) have been widely used to investigate the midbrain dopaminergic system, but only a few studies focused on the functional properties of dopaminergic neurons and their synaptic inputs from dopaminergic and non-dopaminergic neurons also contained in such cultures. In addition, it is not clear whether the culturing process affects the intrinsic neuronal properties and the expression of specific receptors and transporters. We performed patch-clamp recordings from dopaminergic neurons in mesencephalic–striatal co-cultures obtained from transgenic mice expressing green fluorescent protein (GFP) under the tyrosine hydroxylase promoter. Some (10/44) GFP+ neurons displayed a bursting activity that renders the firing of these cells similar to that of the dopaminergic neurons in vivo. The culturing process reduced the hyperpolarization-activated current (Ih) and the expression of D2 receptors. Downregulation of D2 receptor mRNA and protein was confirmed with reverse transcriptase polymerase chain reaction and Western blotting. Immunocytochemistry revealed that many synaptic terminals, most likely originating from dopaminergic neurons, co-expressed the dopamine (DA) transporter and the vesicular glutamate transporter-2, suggesting a co-release of DA and glutamate. Interestingly, exogenous DA decreased glutamate release in young cultures [days in vitro (DIV) < 20] by acting on pre-synaptic D2 receptors, while in older cultures (DIV > 26) DA increased glutamate release by acting on α-1 adrenoreceptors. The facilitatory effect of DA on glutamatergic transmission to midbrain dopaminergic neurons may be important in conditions when the expression of D2 receptors is compromised, such as long-term treatment with antipsychotic drugs. Our data show that midbrain OCs at DIV > 26 may provide a suitable model of such conditions. Citing Literature Volume33, Issue9May 2011Pages 1622-1636 RelatedInformation
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