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Nucleotide sequence of the surface exclusion genes traS and traT from the IncF0 lac plasmid pED208

1986; American Society for Microbiology; Volume: 166; Issue: 3 Linguagem: Inglês

10.1128/jb.166.3.713-721.1986

1098-5530

B. Brett Finlay, W Paranchych,

Microbial Community Ecology and Physiology

pED208 is a 90-kilobase conjugative plasmid belonging to the incompatibility group IncF0 lac. The surface exclusion system from this plasmid was cloned and sequenced, and two genes demonstrated exclusion ability. traS encoded a 186-amino-acid hydrophobic protein which, when transcribed from a vector promoter, caused exclusion of pED208. The product of traT (TraTp) was a 245-residue protein which was highly expressed independently of a vector promoter in Escherichia coli minicells. The TraTp from pED208 was homologous with traT products from the IncF plasmids R-100 and F (80% homology), but recombinants containing the pED208 surface exclusion system excluded F poorly.