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Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Specific Responses to Salinity Stress in Arabidopsis and Rice

2012; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-61779-986-0_18

1940-6029

Aurélie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, Alexander Johnson,

Plant Reproductive Biology

Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.