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Purification and immunological characterization of a GroEL-like protein from Bordetella pertussis

1991; American Society for Microbiology; Volume: 59; Issue: 4 Linguagem: Inglês

10.1128/iai.59.4.1417-1422.1991

1098-5522

Drusilla Burns, Jeanine Gould-Kostka, Martin Kessel, Juan Arciniega,

Escherichia coli research studies

A GroEL-like protein from Bordetella pertussis was purified. This protein was found to have the tetradecameric subunit structure typical of the GroEL family of proteins and to contain epitopes similar to those of other members of this family, including a human GroEL-like protein. Active immunization of neonatal mice with the B. pertussis GroEL-like protein provided little protection against an aerosol challenge with B. pertussis. Antibodies to this protein were elicited in mice by a standard diphtheria-tetanus-pertussis (DTP) vaccine but not by an experimental acellular pertussis vaccine. Since the Bordetella GroEL-like protein was found to contain epitopes similar to those on the mammalian analog, the potential exists that vaccination with standard DTP vaccines may induce antibodies which react with the mammalian GroEL analog.